Vitamin B12

Cyanocobalamin is the most widely utilized form of Vitamin B12 in research and pharmaceutical applications. This water-soluble compound functions as a precursor to the biologically active coenzyme forms methylcobalamin and adenosylcobalamin, which play critical roles in nucleic acid metabolism, fatty acid synthesis, and amino acid metabolism. Cyanocobalamin is a coordination complex with cobalt at its center, bound to a corrin ligand and benzimidazole base. Unlike naturally occurring B12 forms found in animal tissues, cyanocobalamin is synthesized through bacterial fermentation followed by chemical modification, producing a stable, shelf-stable compound suitable for research applications. The 10mL solution is formulated for research use in in vitro cellular investigations examining B12-dependent metabolic processes, mitochondrial function, and neurological signaling cascades in cell culture systems.

Chemical Structure and Identification

• Compound Name: Cyanocobalamin (Vitamin B12)
• Molecular Formula: C₆₃H₈₈CoN₁₄O₁₄P
• Molecular Weight: 1355.38 g/mol
• CAS Number: 68-19-9

Research Applications

• DNA Synthesis and Cell Division: Cobalamin cofactor activity in methyltransferase and mutase enzymes in cell culture [1]
• Fatty Acid Metabolism: Beta-oxidation pathway support and mitochondrial energy production in cellular studies [2]
• Amino Acid Metabolism: Homocysteine conversion and protein synthesis regulation in cultured cells [1]
• Neurological Function: Myelin synthesis and nervous system support mechanisms in in vitro models [3]
• Red Blood Cell Maturation: Erythropoiesis and hematopoietic cell development in cell culture [1]
• Metabolic Cofactor Activity: Methylmalonic acid and propionate metabolism in laboratory research [2]

Reference Citations

1. Vitamin B12 as a precursor for methylcobalamin and adenosylcobalamin | https://pubmed.ncbi.nlm.nih.gov/
2. Sigma-Aldrich – Cyanocobalamin biochemical characterization | https://www.sigmaaldrich.com/
3. Santa Cruz Biotechnology – B12 coenzyme roles in myelin synthesis | https://www.scbt.com/p/vitamin-b12-68-19-9
4. USP Monograph – Cyanocobalamin pharmaceutical standard | https://pubmed.ncbi.nlm.nih.gov/

Important Regulatory Notice

These products are for research use only and are not intended for human consumption, therapeutic use, or diagnostic purposes. All compounds described herein have not been approved by the FDA for human use. These products are strictly intended for in vitro laboratory research and analytical purposes when conducted by qualified research professionals in licensed facilities. Users are responsible for ensuring compliance with all applicable laws regarding purchase and use of these materials.